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Product Overview

Wayne293™ Transfection Medium,Liquid

SKU#: A21501

Size: 1000mL

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Product Description

Wayne293™Expression Medium is a protein-free medium specifically developed for the supporting the growth and transfection. It is compatible and applicable to cell lines HEK293, HEK293T, HEK293A, and HEK293FT under suspension culture conditions. Wayne293™ Expression Medium is a complete, ready-to-use and animal-origin free medium.


Product  Procedure


Product Components

L-glutamine 4mM
Glucose 6.0 g/L
Hypoxanthine & Thymidine No
Phenol red No
Sodium bicarbonate Yes
Hydrolysate Non-animal origin Pepton


High-density value-added:


The growth of different HEK293 cells in Wayne293™. Batch, 125mL shake flask, working volume 30mL, ф25mm, 125rpm, humidity 75%, 5% CO2, 37°C for 7 days.  


Continuous passage growth and transient expression of HEK293 F cells in Wayne293™. 125mL shake flask, working volume 30mL, ф25mm, 125rpm, humidity 75%, 5%CO237℃ culture; passage the next day, 20 passages in total, cryopreserved every 5 generations; unified recovery after passage, and transfection according to the standard method of instruction An IgG plasmid to detect the amount of transient expression.


High yield expression:


Growth and expression of HEK293 F cells after transfection in Wayne293™ and other commercial media. Control: Medium F is supplier G's HEK293 transient medium; 125mL shake flask, working volume 30mL,ф25mm, 125rpm, humidity 75%, 5% CO2, 37℃ culture; according to the standard method of the instruction transfect three expressions respectively Different IgG plasmids were cultured for 7 days.

24 hours after transfection of HEK293F cells in Wayne293 TM and other commercial media



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