
Product Description
QuaCell® HEK293 CD Medium provides a rich environment for high density HEK293 cell growth. QuaCell®HEK293 CD Medium is a serum-free, animal component-free, chemically defined medium developed for HEK293 suspension culture for expression of antibodies and protein products. HEK293 suspension cultures can be subculture directly into QuaCell® HEK293 CD Medium from serum-supplemented or serum-free medium with little or no adaptation. QuaCell® HEK293 CD Medium is formulated without hypoxanthine, thymidine and L-glutamine, suitable for DHFR, glutamine synthetase (GS System) screening system.
Product Components
L-glutamine | No |
Glucose | 6.0 g/L |
Hypoxanthine & Thymidine | No |
Phenol red | No |
Sodium bicarbonate | No |
Hydrolysate | No |
Figure 1. Performance of HEK293T cells in Batch culture of QuaCell® HEK293CD media and commercial medium (Medium EX). 125 mL shake flask, working volume 125 mL, φ26 mm, 125 rpm, humidity 75%, CO2 5%,cultured at 37 ° C for 10 days. When the glucose is less than 3 g/L, it is supplemented to 6 g/L.
Figure 2. Protein production of a recombinant protein-expressing HEK293T cell after 10 days of batch culture in QuaCell® HEK293CD and commercial medium EX. 125 ml shake flask, working volume 30 ml, φ26 mm, 125 rpm, humidity 75%, CO2 5%, cultured at 37 ° C for 10 days. When the glucose is less than 3 g/L, it is supplemented to 6 g/L.
Certificate of Analysis (COA)
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Protocol & Documents

QuaCell® HEK293 CD Medium, Powder

QuaCell® HEK293 CD Medium, Liquid