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QuaCell® HEK293 CD Medium, Powder

SKU#: A22001-10/-100/-XX

Size: 10L/100L/customize

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Product Profile

 QuaCell® HEK293 CD Medium provides a rich environment for high density HEK293 cell growth. QuaCell® HEK293 CD Medium is a serum-free, animal component-free, chemically defined medium developed for HEK293 suspension culture for expression of antibodies and protein products. HEK293 suspension cultures can be subculture directly into QuaCell® HEK293 CD Medium from serum-supplemented or serum-free medium with little or no adaptation. QuaCell® HEK293 CD Medium is formulated without hypoxanthine, thymidine and L-glutamine, suitable for DHFR, glutamine synthetase (GS System) screening system.

 

Product Components


 L-glutamine

No

 
 
 
 

Glucose

6.0 g/L

     Hypoxanthine & Thymidine      No

Phenol red

No

     Sodium bicarbonate      No

Hydrolysate

No


Figure 1. Performance of HEK293T cells in Batch culture of QuaCell® HEK293CD media and commercial medium (Medium EX). 125 mL shake flask, working volume 30 mL, φ26 mm, 125 rpm, humidity 75%, CO2 5%,cultured at 37 °C for 10 days. When the glucose is less than 3 g/L, it is supplemented to 6 g/L.

Figure 2. Protein production of a recombinant protein-expressing HEK293T cell after 10 days of batch culture in QuaCell® HEK293CD and commercial medium EX. 125 mL shake flask, working volume 30 mL, φ26 mm, 125 rpm, humidity 75%, CO2 5%, cultured at 37 °C for 10 days.When the glucose is less than 3 g/L, it is supplemented to 6 g/L.

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