QuaCell® CHO Feed02 Supplement, Liquid
QuaCell® CHO Feed 02 Supplement is a serum-free, animal component-free, chemically defined feed medium. This product provides essential nutrients in the later period of CHO cell suspension culture to maintain the activity, increase the density and improve the protein expression of CHO cells.
|Hypoxanthine & Thymidine||No|
Figure1. Fed-Batch performance of three cell lines in QuaCell®CD04+Feed02 and other commercial media combinations.
Cell line: K1 (hIgG), CHO-S (hIgG), K1-SV (FC-Fusion Protein); control: Combination A is the medium and feed of supplier G, Combinational B is the medium supply of supplier G Commercial H feed; 12mL shake flask, working volume 30mL, ф25mm, 125rpm, humidity 75%, 5% CO2, 37 ℃ for 14 days.
Refer to the respective instructions for the feeding strategy, and when the glucose is less than 3g/L, supplement to 6g/L.
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