Product Description
QuaCell® CHO CD02 provides a rich environment for high-density CHO cell growth. QuaCell® CHO CD02 is a serum-free, animal component-free, chemically defined medium developed for CHO suspension culture for the expression of antibodies and protein products. CHO suspension cultures can be subculture directly into QuaCell® CHO CD02 from a serum-supplemented or serum-free medium with little or no adaptation. QuaCell® CHO CD02 is formulated without hypoxanthine, thymidine, and L-glutamine, suitable for DHFR, glutamine synthetase (GS System) screening system.
Product Components
| L-glutamine | No |
| Glucose | 6.0 g/L |
| Hypoxanthine & Thymidine | No |
| Phenol red | No |
| Sodium bicarbonate | Yes |
| Hydrolysate | No |
Figure3. Fed-Batch performance of common cell lines in QuaCell®CD02. Cell line: K1 (h igG), CHO-S (h IgG), K1-SV (FC-FusionProtein); 125mL shake flask, working volume 30mL, ф25mm, 125rpm, humidity 75%, 5% CO2, 37℃ culture 14 day. Refer to the feeding strategy of QuaCell®Feed02 (Cat.#A11902) instruction manual, and when the glucose is less than 3g/L, supplement to 6g/L.
Certificate of Analysis (COA)
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Protocol & Documents
