QuaCell® Fetal Bovine Serum (FBS), Charcoal Stripped
Product Profile Charcoal stripped fetal bovine serum has been absorbed with activated carbon that removes non-polar material such as lipophilic (lipid-related) materials (virus, certain growth factors, hormones and cytokines) regardless of molecular weight but has little effect on salts, glucose, amino acids, etc.It provides a more defined material for elucidate the effects of hormones in a variety of in vitro systems. Studies include steroid- receptor binding, steroid regulation of cellular receptors, hormone secretion of various tissues and the function of thyroid hormones.QuaCell® Charcoal Stripped FBS is processed on the basis of Fetal Bovine Serum (FBS), Qualified (QuaCell Cat.# B21001). The production procedure includes the use of charcoal and dextran to remove the hormones from the FBS.
- Filtration: 100 nanometer filtration
- Source: Uruguay, South America
- Grade: Fetal cattle
- Endotoxin: ≤5EU/ml
- Double time (MCF-7): ≤30h
- Single cell growth rate: ≥20%
Figure 1. UPLC results of FBS samples before and after DCC stripping treatment. Thus it can be seen that various growth factors, hormones and cytokines in FBS were effectively reduced after DCC stripped.
Figure 2. Growth test results of hormone-sensitive MCF-7 cells. Group 1 (Untreated FBS+ IGF-1)Stimulating rapid proliferation of MCF-7 cells. Group 2 (DCC Stripped FBS+ IGF-1): MCF-7 cells grew at normal rates. Group 3 (DCC Stripped FBS without IGF-1): MCF-7 cells grew slowly. Base Medium: MEM (Phenol red free,with NEAA and Glutamine). Additive：0.01 mg/ml recombinant human IGF-1 Untreated / DCC Stripped FBS to a final concentration of 10%. Atmosphere: air, 95%; CO2, 5%. Temperature:37°C. The results showed that the contents of insulin and estrogen were effectively reduced in FBS stripped by DCC.
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