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QuaCell® CHO Feed02 Supplement, Liquid
SKU#: A11902
Size: 1 Set (1000mL FeedA+100mL FeedB)
Product Description
QuaCell® CHO Feed 02 Supplement is a serum-free, animal component-free, chemically defined feed medium. This product provides essential nutrients in the later period of CHO cell suspension culture to maintain the activity, increase the density and improve the protein expression of CHO cells.
Product Components
| L-glutamine | No |
| Glucose | 50.0 g/L |
| Hypoxanthine & Thymidine | No |
| Phenol red | No |
| Sodium bicarbonate | No |
| Hydrolysate | No |
Figure1. Fed-Batch performance of three cell lines in QuaCell®CD04+Feed02 and other commercial media combinations.
Cell line: K1 (hIgG), CHO-S (hIgG), K1-SV (FC-Fusion Protein); control: Combination A is the medium and feed of supplier G, Combinational B is the medium supply of supplier G Commercial H feed; 12mL shake flask, working volume 30mL, ф25mm, 125rpm, humidity 75%, 5% CO2, 37 ℃ for 14 days.
Refer to the respective instructions for the feeding strategy, and when the glucose is less than 3g/L, supplement to 6g/L.
Certificate of Analysis (COA)
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Protocol & Documents
QuaCell® CHO CD04 Medium, Liquid
SKU#:
A11004
Size:
1000mL
QuaCell® CHO CD04 Medium, Powder
SKU#:
A12004
Size:
10L / 100L / Customized
QuaCell® CHO CD02 Medium, Powder
SKU#:
A12002
Size:
10L
QuaCell® CHO CD02 Medium, Liquid
SKU#:
A11002
Size:
1000mL
QuaCell® CHO Feed02 Supplement, Powder
SKU#:
A12902
Size:
3L / 10L / Customized
